Journal: Journal of Cancer
Article Title: The oncogenic and prognostic role of PDK1 in the progression and metastasis of ovarian cancer
doi: 10.7150/jca.47278
Figure Lengend Snippet: PDK1 is overexpressed in ovarian cancer. (A) Expression of four isoforms of PDK in ovarian cancer cell lines, CAOV3 and SKOV3, and normal ovarian epithelial cell line, HOSEpiC. (B) PDK1 was highly expressed in CAOV3 and SKOV3 cells compared with HOSEpiC cells. (C) PDK1 was highly expressed in ovarian cancer tissues compared to benign ovarian tumors. (D) Immunohistochemical detection of PDK1 protein expression in paraffin-embedded tissues. Positive PDK1 staining was observed mainly in the cytoplasm of OC tissues; a, IHC scoring = 0; b, IHC scoring = 2; c, IHC scoring = 3; d, IHC scoring = 4; e, IHC scoring = 6; f, IHC scoring = 8; g, IHC scoring = 9; h, IHC scoring = 12; a and b, PDK1 was not detected in OC tissues; c and d, representative images of weak PDK1 staining in OC tissues; e and f, representative images of moderate PDK1 staining in OC tissues; g and h, representative images of strong PDK1 staining in OC tissues; original magnification ×200.
Article Snippet: The normal cell line used in this study was human ovarian surface epithelial cell line (HOSEpiC) obtained from ScienCell Research Laboratories (San Diego, CA).
Techniques: Expressing, Immunohistochemical staining, Staining